The examined fish intoxicated with aflatoxin B₁ (AFB₁) showed several non-specific signs, including inadequate growth, significant losses in aquaculture industry especially resulting from aflatoxicosis. The study using Zinc oxide (ZnO) and Hematite (α-Fe₂O₃) nanoparticles (NPs) to assess the effect of using as on their own negative impacts of AFB₁ 3 ppm/ Kg for 13 weeks was studied 360 healthy fingerlings of Nile tilapia, were divided into eight groups (15 fish/ group) with three replicates, it has been applied in 24 aquariums. Group < sub>1 (G₁) fed only a basal diet as control. While, G₂, G_3, and G₄ were fed on diets supplemented with 2 g/kg diet of ZnO NPs, Hematite NPs and combination of both respectively. Group < sub>5 (G₅) was fed on diets contamination with AFB₁ (3 ppm/kg diet), while G₆, G₇ and G₈ were fed on diets containing 2 g/ kg diet of (ZnO NPs, Hematite NPs and combination of both) plus AFB₁ (3 ppm/kg diet) respectively. Experimental treatments affected significantly on the Final Body Weight (FBW), Daily Gain (DG), Feed Conversion Ratio (FCR) and mortality Table (1, 2(. FBW and DG reduced to 37.856 g and 0.227 g in fish feed containing AFB₁, when compared with groups AFB₁ which supported with feed additives (ZnO, Hematite and combination of both) NPs, which reported final body weight 45.507, 47.007, 49.762 g and daily gain 0.313, 0.328, 0.360 g respectively. The highest FBW and DG were determined in group treated with feed additives combination of (ZnO and Hematite) nanoparticles, keep it track of by the fish group fed diet supplemented with Hematite NPs then those fed diet supplemented with ZnO NPs and the recorded values were for final body weight 55.857, 61.500, 63.874 g daily gain 0.427, 0.488, 0.515 g respectively than that of the control group G₁ 52.784, g FBW and 0.393 g DG. The highest survival rate was obtained in fish group treated with combination of (ZnO and Hematite) nanoparticles, The survival rate was (95.556 %) in fish fed diets combination of (ZnO and Hematite) NPs, while it was 57.778 % in fish group G₅ fed AFB₁, which enhanced in aflatoxicated group G₈ which supplemented with combination of (ZnO and Hematite) NPs 84.444 %, and in aflatoxicated group G₇ which supplemented with Hematite NPs 77.778%,  and in aflatoxicated group G₆ which supplemented with ZnO NPs 75.556%. The best FCR was obtained in same order in fish group treated with combination of ZnO NPs and Hematite NPs, Hematite NPs then ZnO NPs 1.187, 1.170, 1.253 compared to the control group which recorded 1.462. On the other hand, aflatoxicated groups significantly affected feed conversion impaired significantly affected, aflatoxicated group recorded 1.950 but when supported with ZnO NPs, Hematite NPs and combination of both recorded 1.541, 1.525 and 1.502 respectively.