Nigella sativa, L. plants were fertilized with NPK or 5, 10 and 15 m3/fed. Chicken manure. Inorganic and organic fertilizer represented the main plot. The sub plot treatments were royal jelly (200 and 300 ppm) salicylic acid (100 and 200) and active dry yeast (5 and 10 g/l) as well as control. The obtained results showed the following black cumin fertilized with mineral NPK exhibited the best vegetative growth parameters and the highest number of capsule / plant, seed yield / plant and / fed., volatile oil % and yield / plant and / fed., fixed oil (% and yield / plant and / fed.), as well as, N, P and K % in the leaves. Chicken manure treatments significantly decreased all previous parameters as compared with mineral NPK, except high level of chicken manure (15 m3/fed.) which resulted the lowest reduction without significant differences between it and mineral NPK treatment. All spraying treatments significantly increased all previous parameters over the untreated (control). Among six used treatments, the treatments of royal jelly (300 ppm) followed by active dry yeast (10 g/l) then salicylic acid (200 ppm) resulted the highest values of vegetative growth traits and seed yield ,as well as, oil yield (volatile and fixed) and P % in the leaves. Active dry yeast at 10 g/l gave the highest percentages of N and K in the dry leaves comparing with other treatments. The interaction between main-plot and sub-plot was the most effective on the previous parameters. The highest values in all measurements were obtained due to mineral NPK or high level of chicken manure (15 m3/fed.) in combination with royal jelly (300 ppm), active dry yeast (10 g/l) or salicylic acid (200 ppm).