A pot experimental study was conducted  at the Floriculture Nursery of the Horticulture Department, Faculty of Agriculture Moshtohor, Benha University, during 2014 and 2015 seasons to study the effect of 20 treatments which were represented by the combinations between five different growing media, i.e. sand + clay, sand + clay + perlite, sand+ clay + compost, sand + clay + peatmoss, and compost+ perlite+ peat moss (1:1:1 by volume) and four chemical fertilization rates of kristalon fertilizer at 0, 2,4 and 6g/L on the growth and chemical composition ofCupressus macrocarpa plants. Results showed that growingCupressus macrocarpa plants in a mixture medium contained compost + perlite+  peat moss (1:1:1 by volume) + kristalon fertilizer at 6g/L  or 4gL produced the tallest plant, the highest values of fresh and dry weights of shoots and, root length (cm),number of roots/plant and fresh and dry weights of roots/plant in both seasons. Besides, the highest leaf total nitrogen, phosphorus, potassium contents . In addition, the greatest total carbohydrates content of Cupressus macrocarpa was recorded by using the mixture media of compost +perlite+  peat moss  and sprayed  Kristalon fertilizer at 6g/L .In this context, The medium consists of compost+pearlite+peatmoss and sprayed with  kristalon fertilizer  at 4 g / L came second in relation to the above-mentioned studied parameters  in both seasons. Conclusively, growing Cupressus macrocarpa plant  in a medium contained compost+  perlite+ peat moss and sprayed with kristalon fertilizer at 6g/L produced the best growth and quality of lemon cypress (Cupressus macrocarpa) plants.