Using propolis ethanol 70% extract on the growth of three bacterial species showed that by increasing extract concentration, the zone of growth inhibition increased in the three tested bacterial species where, at 8 ppm the growth inhibition zones were 14.3, 21.3 and 30.0 mm for Bacillus subtilis, Escherichia coli and Klebsiella pneumoniae, respectively. On the other hand the lowest concentration (2ppm) showed the lowest growth inhibition for all bacterial species (9.3, 15.3 and 22.6 mm, respectively). The medium concentration (4ppm) was intermediate. However, propolis-water resulted no growth inhibition except for the B. subtilis which was affected by 4 and 8 ppm; (4.3 and 8.0 mm extract growth inhibition respectively).