This work focused on the production and characterization of peroxidase enzymes (lignin peroxidase (LiP), manganese peroxidase (MnP) and versatile peroxidase (VP)) from Pleurotus sapidus in the submerged cultivation. Among four tested media, basal medium containing 1% rice straw was the best for maximum peroxidases secretion by P. sapidus. Wheat straw was the most suitable carbon source for the production of peroxidases. Concentration of 15 g/L of wheat straw was the most suitable for maximum production of LiP and MnP. While, VP showed maximum productivity at 20 g/L of wheat straw. Maximum specific activities of LiP and MnP enzymes were at 2g/L of yeast extract. While, concentration of 2.5 g/L of yeast extract gave the highest secretion of VP by P. sapidus. The highest specific activities of LiP and VP were obtained after 12 days of incubation. While, MnP reached the maximum specific activity after 3 days of incubation. The optimum temperature for LiP and VP specific activities was 60°C. In addition, MnP showed its optimum temperature at 50°C. Concerning the optimum pH, LiP reached the maximum activity at pH3. Moreover, pH 5 and pH3.5 were the optimum for the activity of MnP and VP, respectively.