Two field experiments were conducted at Nubariya Research Station, Cotton Research Institute, Agricultural Research Center Giza, Egypt during the two summer growing seasons, 2017 and 2018. The aim of the present investigation was to study the effect of bio-fertilization Cyanobacteria extract, plant Growth promoting Rhizobacteria (PGPR) extract and Tea compost, nano-fertilization (NPK mixing) and their combination among 50% nano fertilization+ PGPR extract, 50%Nano fertilizers+ tea compost, 50% Nano +Cyanobacteria extract and 50% Nano+ PGPR extract+ tea compost+ Cyanobacteria extract and untreated one (control) on growth, seed yield , lint yield , and fiber properties of Egyptian cotton Giza 94 cultivar. The most important results can be summarized as follows: the combined treatment  which contains half dose of both the nano-fertilizer and the some biofertilizers gave the highest values for, i.e. plant height (cm), number of fruiting branches per plant, number of open bolls per plant, boll weight (gm), lint percentage, seed index, seed cotton yield (Kentar/fed.), lint cotton yield (Kantar/fed.), micronaire value, fiber maturity ratio (MR), upper half mean (UHM)mm, fiber uniformity index (UI), and fiber strength  (g/tex), followed by the treatment of 100% nano then the recommended dose of mineral fertilizer NPK. On the other hand, the lowest values of all the above traits, were recorded from the half does of nano NPK fertilizer in 2017 and 2018 seasons, respectively .While, There were no significant differences between the means of the fiber elongation % due to the treatments applied during the study in 2017 season .It's worthily to mention that, the percentage of fiber elongation had no constant trend due to fertilizer transactions applied on the adopted genotype during` 2018 season. But, it seems to be that nano-fertilizer is boosting the fiber elongation percent.