This study was conducted during 2013 and 2014 season's atfisha village, Mansoura Governorate to improve fruit quality of Anna apple trees. Treatments included that mixture₁ content (Biolab) at 1 ppm {low concentration}; mixture₁ with high concentration at 2 L/ 600 liters water; mixture₁(Low+ high) concentrations; mixture₂content (Anti-stress) at 1 ppm; mixture₂with high concentration at 2 L/ 600 liters water; mixture₂ (low+ high) concentrations; Pepton at 1 ppm; Pepton with high concentration at 2 g/ liter water and control. All treatments sprayed three times at the mid of March, April and May. Results indicated that, mixture₂ (low+ high) concentrations and Pepton treatments gave the best significant fruit number per tree. Mixture₂ (low+ high) concentrations; pepton and mixture₁ indicated the highest significant yield. Mixture₁ gave the highest significant fruit weight. Acidity percentage decrease significantly with bio-compounds treatments than the control. Mixture₂ (low+ high) concentrations; mixture₁ with high concentration and mixture₂ had more pronounced positive affect on fruit color. The highest significant total phenols with control treatment. Total indoles were the highest with pepton. Total protein were the highest mixture₂._Leaves SDS-PAGE revealed total of 24 common bands (monomorphic bands) were detected while, the remaining twenty bands were polymorphic with 45.45% polymorphism. This variation was found between present or absent bands with different treatments may be due to the differences between improving fruit quality of Anna apple cultivar. Isozymes bands with poly phenol oxidase and peroxidase and were varied between treatments under study. It can be recommended that spraying with bio compound are improving fruit quality of Anna apple cultivar.Mixture₂ {low and high} with concentration {1.0 ppm+ 2 L /600 liter water}; Mixture1 with concentration 1 ppm and pepton with concentration 1000ppm are improving yield and fruit quality.