Tow field experiment were carried out during two successive summer seasons 2013 and 2014 at the Experimental Station at Khamisalocation Siwa Oasis, Desert Research Center(DRC), to study, response of soybean (Glycine max) Giza, 35 cultivar to the combination between phosphorus fertilization, and four levels phosphorus fertilization:(0, 30, 45and60 kg. P₂o₅ / fed.). And four spring with four bio-fertilizers treatment (control, bio magic compound at a rate of (2 liters / fed.), amino carpi K compound (200 cm³ / fed.), and Bio greenmeracl compound (1 liters / 200 liters water / fed.).Using the split plot design which, the bio-fertilizers treatment were distributed in the main plots, whereas phosphate fertilization in subplot in four replicates.   The obtained results could be summarized as follows: The results showed a significant increase in yield and its components, as well as the oil yield / fed. using ground phosphorus fertilization at the rate of 60 kg p₂o₅/fed. in both seasons respectively. The three bio- fertilizers of significantly differences were significant and increased the yield and its components as well as the oil crop, and the best bio-fertilizer was a compound Amino Kirby K, 200 cm³, in both seasons. The interaction between phosphorus and bio- fertilizers led to increasing the yield and its component. The best practice at a rate of 60 kg p₂o₅/fed. + spraying with Amino Kirby K, 200 cm³ /fed., in both seasons.The results showed that phosphorus fertilization at the rate of 60 kg p₂o₅/fed.  and spraying with Amino Kirby K, 200 cm³/ fed.,resulted in significant increase in the chemical components of soybean seeds namely: nitrogen, total protein,oil percentage, phosphorusand potassium, in both seasons.Found from the calculation of the economic yield of crop and oil yield, the use of the high rate of phosphorus fertilization (60 kg. P₂o₅ /fed.) and spraying with amino carpi K compound (200 cm³ / fed.) is the best experimental economic transaction for the farmer under the conditions of Siwa Oasis, especially that the crop of soybeans is grown for the first time in the oasis.