This study was conducted to evaluate the inhibitory activity of two types of natural honey ( citrus flowers honey and eucalyptus honey) and two types of commercial honey available in the market (sinbola honey and shafi honey), on growth of Escherichia coli, Pseudomonas aeruginosa. and their sensitivity to antibiotics Amikacine (AK) 30μg, Ampicillin / Sulbactum (SAM) 20μg, Augmenten (AUG) 30μg, Chloramphenicol (C) 30μg and Gentamicin (GM) 10μg. The results showed that the natural honey significantly superiority compared to commercial honey on inhibiting bacterial growth for isolates. The diameter of inhibition zone of citrus flowers honey and eucalyptus honey against E. coli growth was 20 and 21 mm, respectively and P. aeruginosa was 19 and 14 mm, respectively compared to commercial sinbola honey which gave an inhibition diameter of E. coli 8 mm while not affected on P. aeruginosa , as for shafy honey it not affected in bacterial isolates growth. Also the results showed that the citrus flowers honey exceeded significantly compared to eucalyptus honey for P. aeruginosa the inhibition diameters of citrus flowers honey and eucalyptus honey were 19 and 14 mm respectively. On the other hand, the results showed that the inhibitory effect of natural honey was closely related to antibiotics, and it gave a positive result when compared to the standard tables of the inhibition of antibiotics. P. aeruginosa was resistant to both chloramphenicol, Augmenten and Ampicillin, while sensitive for two types of natural honey. The results indicated that the combination of natural and commercial honey with antibiotics was increased the efficiency of antimicrobial activity of antibiotics by increasing the diameters of bacterial growth inhibition compared with the diameters which given by antibiotics. The citrus flower honey had the greatest inhibitory effect on bacterial isolates when it was mixed with antibiotics followed by Eucalyptus honey, while commercial sinbola honey was the least impact.