Two field experiments in randomized complete Block design (RCBD) were conducted at the experimental farm, Faculty of Agriculture Moshtohor, Benha university, Egypt during 2016 and 2017 seasons to study the effect of Putrescine at 5 and 10 mg/L, Benzyl adenine (BA) at 20 and 40 mg/L and yeast extract at 50 and 100 ml/L on growth, photosynthetic pigments, phytohormones content, anatomical structures, flowering, characteristics; seed fuelling and yield of Soybeans (Glycine max L.) plants. Results showed that different applied treatments increased growth characteristics i.e., (plant height, leaf number, leaf area, branches number, fresh & dry weights as well as the photosynthetic pigments and the endogenous phytohormones content. Also, stem and leaf anatomy measurements were increased especially (stem and leaf vascular and transporter tissues. In addition, flower setting was increased with putrescine, BA and yeast extract during 2016 and 2017 seasons. Also, yield and its characteristics were increased especially upper fully seeds, seed yield weight/plant during both seasons.