The collected water samples were microbiologically evaluated, the total bacterial count was evaluated at 22°C and 37°C. Total bacterial counts at different seasons of investigations revealed that 37^oC obtained higher rate of bacterial counts than at 22^oC. Total coliform was also recorded for water samples that obtained directly from the River Nile during all examination periods. There was no E. coli contamination found in the final treatment stage and all distribution regions. No fecal streptococci contamination was found in the final treatment stage and all distribution regions. The highest Salmonella and Shigella counts were recorded for water samples obtained directly from the River Nile during all examination seasons. On the other hand, Salmonella and Shigella were not detected in all distribution regions. Higher value of turbidity was observed during the summer period followed by the spring in both seasons. During the investigation period pH values ranged between 7.0 to 8.4. The highest pH value was recorded during winter 2017. Whereas, the lowest value was recorded during autumn 2016. The lowest COD values were recorded in summer 2016 and winter 2017. Whereas, the highest COD values were recorded in summer 2017 for water samples that obtained directly from the River Nile during all examination seasons. The highest BOD value (15.2 mg/l) was recorded in winter 2017 for water samples that obtained directly from the River Nile during all examination seasons.