This work was carried out at the sandy soil of El-Khatatba region, Minofia Governorate, Egypt, through the two successive seasons of 2016 and 2017 to study the effect of organic fertilization combined with chemical fertilizer in presence of bio fertilizer on growth, yield, quality and nutritional status of banana Grande Naine cultivar. Mother plants were planted at 3×2 m apart in March 2015 and two suckers were selected per each hole and the others were removed. The obtained results showed that, the highest values of pseudostem height, pseudostem circumference, leaf area and assimilation area per plants were scored by T1 recommended dose of chemical fertilization (RD), followed in descending order by T4 treatment (75% RD+21kg compost+5ml bio./plant) with non-significant differences between them in the two seasons. The highest values of bunch length, fruits yield per fed., the thickest bunch, the heaviest bunch, the highest number of fingers/hand and the heaviest hand were scored by T1 treatment, followed in descending order by T4 treatment in the two seasons. The highest values of finger length, finger diameter and finger weight as well as the richest fruit pulp total sugars % and T.S.S. % were recorded by T4, followed by T7 (75% RD+45g humic+ 5ml bio./plant) in the two seasons. The heaviest pulp weight was obtained by those received T4 treatment, followed in descending order by T7 treatments, whereas the highest pulp/peel ratio was registered by T7 and T4 treatments in the two seasons. Banana plants received the full dose of chemical fertilization (100% RD) is being the most promising one for inducing the greatest leaf total carbohydrates, N, P and K percentage, followed in descending order by T4 and T7 treatments without significant difference between them in the two seasons.