The possible effects of β-carotene orally supplementation upon gestational stages changes in serum concentrations across time for total protein (TP), albumin (ALB), globulin (GLB), total cholesterol (TC), triglyceride(TG) and alanine transaminase(AlT) and aspartate transaminase(AST), Haemoglobin (Hb), Red blood cells count (RBCs), packed cell volume (PCV) malondialdehyde(MDA),reduced glutathione(GSH), total antioxidant capacity(TAC) and progesterone (P4) were evaluated. Experiments were carried out from May to October and were constructed into two groups of gestational goats (20 does each) with 2-3 years (body weight 26±2kg) crossbred does50%Zaraibi x 50% Baladi. The first group was considered as control (C) while the other one was β-carotene (BC) group in which 50 mg BC was oral supplemented/day/goat. Serum blood samples were collected in order to quantify progesterone concentrations (P4) through radioimmunoas­say. TP, ALB, GLB, TC, and TG were analyzed spectrophotometrically. Oral supplementation with β-carotene significantly increased (p ≤ 0.05) the concentrations of TP, ALB,GLB, GSH,TAC and P4 with the associated significant decrease in TC, TG and MDA concentrations, compared with the control. Gestation stages showed significant differences (p ≤0.002and p ≤0.05) in all biochemical parameters, haematological, oxidative stress parameters and P4 level.  However, a treatment × gestation stages interaction occurred between treatments for TP, ALT, AST, Hb, RBCs, PCV, MDA, GSH, TAC and P4 (P < 0.002 and p ≤0.05) favoring the β-carotene group.