Transmission of pathogenic organisms that cause nosocomial infection to the community can be occur by number of different ways and particularly through health staff, visitors and discharged patients. Isolation and identification of multidrug resistant bacteria (MDR) from various medical specimen at Zagazig University hospitals, Egypt has been reported. The identification of such bacteria morphologically, physiologically and biochemical test were identified as Staphylococcus aureus, P. aeruginosa, K. pneumonia, E. coli, P. vulgaris and E. fecalis. The most effective plant extracts were Tamarindus indica., Rosmarinus officinalis., Hibiscus sabdariffa.,on growth of selected bacteria. Potassium leakage after 100 min of exposure to plants extract induced intracellular potassium ions by 11.3-13.5 ppm and 4.5-8.3 ppm for Staphylococcus aureus and Pesudomonas aeruginosa respectively. selective permeability of the cytoplasmic membrane, and is thus a primary indication of membrane damage The major damage to the bacterial cytoplasmic membrane were measured by leakage of nucleotides after 8 hours of plant extract exposure were record 0.3- 0.48 and 0.2-0.38 nm for Staphylococcus aureus and Pesudomonas aeruginosa respectively.