Identification and characterization of the plants is very important to protection and economical use. This work used the RAPD method for show the molecular characteristics of six species of Asteraceae. The study was carried out from the April 2021 to July 2021. five primers were used to amplify the DNA from the studied species. The obtained results of the RAPD profiles ranged from 100 to 1500bp. About 40 bands were cleared for the studied six species. The number of bands per species varied from 2 to 12 for primer. The highest similarities value was obtained at Sonchus oleraceus and Senecio desfontainei (88%) and the lowest obtained at Bidens pilosa and Cichorium pumilum (61%). The RAPD-PCR dendrogram classified the studied species of into definite clusters according the primer. Primer c separated Cichorium pumilum and Senecio desfontainei in the first clusters as a genetically different about the others in the second cluster, meanwhile primer d separated Urospermum picroides and Cichorium pumilum in definite clusters than others. From this study, it can conclude that, the RAPD can differentiate between the six Aster species, through rapid method to economical use as well as identification and conservation.