Some biological parameter of raw water as the source and treated water in Abassa station,Abu hammad,Sharkia Governarate such as algal count,protozoa,total bacterial count,total coliform and fecal coliform .These results showed that the algal count of algae in treated water ranged between 9 to 36 algal count /1L ,no protozoa , no total coliform and no fecal coliform found in treated water while the count of total bacteria found in treated water ranged between 0 to 2 CFU/1ml.Different bacterial isolates which isolated from different stages in abassa water station (Raw , Filtrate , Treat, Net) and identified by using some biochemical tests such as Staphylococcus spp,E.coli spp,Proteus spp,Salmonella spp,Bacillus spp,Micrococcus spp,Klebsiella spp .Using natural extracts such as moringa olifera seed,okra seed , clove seed and ginger seed as antibacterial activity against some isolated bacteria and results showed that moringa seed extract was the most effective extract as antibacterial activity with concentration 1.0gm/50ml give 28 mm inhibition zone diameter against Stapylococcus spp .