This study was carried out to evaluate the microbial infections in natural way and reduce the antibiotic resistance. One hundred bacterial isolates were collected from clinical specimens of patients suffering from bacterial infection (55 females and 45 males). The bacterial isolates were obtained from 8 different specimens with the following percentage representations: urine (30.58 %), blood (20%), abscess (20%), wound (9.41%), throat and tube swab (5.88%), ear discharges (5.88%), vaginal swabs (5.88%) and eye swab (2.35%).The results showed that (29.41%) Escherichia coli, (23.53%) Klebsiella pneumoniae, (17.65%) Staphylococcus aureus, (15.29%) Pseudomonas aeruginosa, (8.24%) Proteus vulgaris, (5.88%) Acinetobacter baumannii. Among 17 antibiotics tested, meropenem, a carbapenem antibiotic, was the most effective drug against most of the gram-negative and gram-positive bacteria. Detection of biofilm by two different methods show that most of isolates were multidrug resistant. Three species of lactic acid bacteria namely L.acidophilus (DSM20079), L.plantarum (DSM20174) and L.salivarus (DSM20555) were used for reducing the microbial infections. Bacteriocins were isolated from MRS broth culture of these lactic acid bacteria through the precipitation method using 1N HCL and were tested against different pathogenic bacteria. Antibacterial activity of bacteriocins extracted from L. acidophilus (DSM20079) showed maximum activity against most isolated bacteria as compare to others. Also, L.salivarus (DSM20555) have the highest activity against Methicillin-resistant Staph aureus. The present study has showed the antibacterial role of bacteriocin isolated from lactic acid bacteria can be used for treatment variety of human diseases.