Background: Nursing students are key assets of nursing profession and their attitude has a great influence on their academic achievement motivation and the future quality of nursing.  Aim of the study: was conducted to assess nursing students' attitude toward nursing profession and achievement motivation at Faculty of Nursing - Zagazig University. Subjects and Methods: Research design: A Descriptive design was employed to convey the present study. Setting: The study was conducted at Faculty of Nursing, Zagazig University. Subjects: Representative samples of 324 nursing students were randomly selected from academic year 2018-2019). Tools of data collection: Two tools were used for data collection. Tool (I): The attitude toward nursing profession scale. Tool (II): The achievement Motivation scale. Results: The most of nursing students had a positive attitude toward nursing profession (96 %). While, the least of them (4%) had a negative attitude. Additionally, the most of nursing students had a high level of achievement motivation (99.1 %). Whereas, the least of them had a low level (0.9%).Conclusion: The nursing students' attitude toward nursing profession was positively & significantly correlated to their achievement motivation. Moreover, nursing students' attitude toward nursing profession is a significant predictor of their achievement motivation. Recommendations: The nursing faculty members conduct a conference for newly admitted students to guide them on the nursing profession, nursing history, nursing education programs and various clinical experiences. Additionally, the faculty management should   provide a good infrastructure and institution facilities to the student and nurse educators to ensure a high level of achievement motivation.