The experience of infertility can be extremely stressful and associated with a range of personality traits in infertile women, which have greater susceptibility to depression and anxiety.  This study aimed to assess the personality traits of infertile Yemeni women in comparison with fertile ones with 50 infertile and 100 fertile women attending infertility and family planning clinics in the health center at Al-Mukalla city, in Yemen. Middlesex Hospital Questionnaire (MHQ) was used to assess the personality traits as phobia, depression, obsession, hysteria, anxiety and Psychosomatic symptoms. Results showed that the fertile women had slightly higher scores of psychosomatic symptoms and hysteria compared to infertile ones. Infertile women with no acceptance reaction had higher scores of anxiety, obsession, depression, and hysteria. There are a positive statistically significant correlation between depression and education among fertile women. It is recommended that nurses dealing with infertile women be aware of emotional factors in the problem of infertility, as they can play a vital role in providing emotional support and assisting with effective coping strategies.