The aim of this study was to identify the patient's responses both physically and psychologically to selected invasive procedures (venipuncture, naso-gastric intubation and urinary catheter insertion). A sample of convenience of one hundred adult patients subjected to selected invasive procedure in both medical and surgical wards were recruited for this study. Three tools were utilized to collect data pertinent to the study. A semistructured interview and participant observation techniques were utilized to collect data at El-Manial University Hospital. Frequency and percentage distribution and chi-square test were used for the analysis of data. The study findings showed that the majority of the studied group subjects (medical and surgical patients) exhibited: facial expression of stress, and vocalization of stress (χ2 = 8.98, p<0.05; χ2 = 21.24, p<0.05 χ2 =14.43, p<0.05; χ2 = 136.36, p<0.05 respectively) while non experienced changes in vital sings, sweating and /or pupil dilatation. In relation to patients psychological responses the majority of the subjects reported that they become stressed due to: lack of information about the procedure, the presence of strange persons during the procedure, and absences of health care providers watched the procedure after insertion. Moreover all the study subjects indicate that all the selected invasive procedure were painful. In light of the study results, replication of the study on a larger probability sample, and correlation of the socio-demographic data with the physical and psychological responses were recommended to be investigated.