Background: Brucellosis is a pervasive problem throughout the world, especially in developing countries. It has a great impact on human health and animals, these all affect on social income. Aim of the study: To evaluate the effect of health educational intervention for brucellosis among slaughterhouse workers at Gharbia Governorate. Subjects and Methods: Research design: Aquasi- experimental design was in this study used to collect data. Setting: This study was conducted at four slaughterhouses in Tanta, Kfer El-Zayat, Bassioun and El-Mahala El-Koubra in Gharbia Governorate .Subjects: Eighty five workers' was selected randomized as a study sample. Tools of data collection: Data were collected using three tools. Tool I: A structured interview questionnaire to collect data about slaughterhouse workers. Tool II: An observation checklist, to assess workers practices throughout work process. Tool III: Blood analysis to slaughterhouse workers for brucellosis disease. Results: Results: revealed that the mostly of workers were males, and married, nearly half of them reside rural areas, and more than half of workers reside in urban areas. About three quarter of workers was drinking non boiled milk, and one quarter of them was infected by brucellosis. Slightly more than of workers were veterinaries also more than one quarter from manual workers, less than one fifth from butchers and skinners. Conclusion: One quarter of slaughterhouse workers were exposed to brucellosis infection during work. The training program was effective and increased the level of workers' knowledge and practices about brucellosis disease. Recommendations: A booklet containing information about brucellosis should be available at all slaughterhouses in Gharbia Governorate