We propose and demonstrate experimentally the possibility of reproducing the attenuation coefficients, Young's Modulus, the acoustic reflection coefficients, and the thermal conductivity of a real biological tissue which using 2% Agar based phantom (2g Agar for 100ml degassed water).The design of 2% Agar based phantom fabrication is discussed. The attenuation coefficient is measured using measurements of the acoustic properties and Young's modulus is measured using the mechanical compression test by applying the relation between the Nominal Stress and the Nominal Strain. Phantom is a material that simulates some properties of body tissues. It is important for helping the development of Hand-Eye coordinate necessary for ultrasound guided intervention and also allowing the performance of the simulated interventions without having to do it as the first time in the patient. Acoustic properties were measured for the frequency of 4.8M Hz at a range of ambient temperatures (10 – 35 °C). The acoustic velocity of the TMMs remained relatively constant with increasing frequency.