ABSTRACT
A method was developed for the chromatographic separation and quantification for a mixture of Malathion (as organophosphorus nerve agent), and S-mustard (as blister agent). The method used a high performance liquid chromatography (HPLC) with reversed phase C-18 column, and UV detection at 220 nm. The compounds were separated using isocratic elution acetonitrile-water (50%) at a flow rate of 0.7 mL/min in a period of 5 min. The retention times were (1.939) min for S-mustard and (3.063) min for Malathion. The calibaration curves were linear (R2 = 0.999) in the studied concentration range (200 to 1000 μg/mL). Sensitivity of the method for quantification of Malathion was found to be higher than that of S-mustard by four times. In addition, the accuracy was found to be (~ 97%) for both.