Abstract Pyridine contaminated wastewater presents a great threat on water resources safety. In this study, microwave (MW) radiation was developed for the removal of pyridine from wastewater. The effects of pH, radiation time, post irradiation and initial pyridine concentration on the removal were investigated. The initial pH and radiation time showed significant effects on the removal of pyridine. The largest removal was obtained at pH 9 with initial pyridine-concentration of 20 ppm (MW radiation time 5 min). Wastewater containing high concentration of Pyridine was treated by MW radiation.Up to 97.5% removal. It could be proposed that MW radiation was an effective method for the removal of Pyridine from wastewater.