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29896

Ameliorative Effects of He:Ne Laser Irradiation on DNA Damage of Blood of Children with Acyanotic Heart Disease

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Last updated: 22 Jan 2023

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Abstract

Abstract
Low-energy laser irradiation (LELI) has been found to attenuate various biological
processes in tissue culture and experimental animal models. The aim of the present study was
to investigate the role of LELI in ameliorating markers of oxidative stress (in terms of blood
antioxidant enzymes levels, NO generation, apoptotic genes together with DNA
fragmentation and apoptosis percentage in circulating lymphocytes) in cyanotic and acyanotic
congenital heart diseases with reference to normal healthy children. Accordingly, whole
blood from 16 cyanotic, 14 acyanotic and 15 controls was exposed to 8 mW He-Ne laser at
energy densities of 3 J/ cm2 and examined before, after and without laser exposure 24 hours
post irradiation for levels blood antioxidant enzymes, NO generation, Bax RNA and Bcl2
protein together with DNA fragmentation and apoptosis percentage in circulating
lymphocytes. Results indicated that there were significant increases in mean ± SD of plasma
nitrite, MDA, DNA fragmentation and apoptosis in circulating lymphocytes of both acyanotic
and cyanotic cases compared to controls before laser irradiation. Laser ameliorated oxidative
stress in acyanotic heart disease blood, but increased apoptosis and DNA fragmentation in
cyanotic cases 24 hours post laser irradiation. In control cases laser had a biostimulatory
effect exhibited by decreased lipid peroxidation, apoptosis and DNA fragmentation
compared to its level without laser irradiatioin. Also, there was significant increase in plasma  nitrite in controls, cyanotic and acyanotic cases 24 hours post irradiation with laser compared to their level without laser irradiation. However, cyanotic cases showed the highest
significant value and were significantly higher when compared to both cyanotic and acyanotic
cases. It is concluded that He:Ne laser have an ameliorative effect on damaging effects
induced due cardiac catheterization procedure in case of acyanotic congenital heart diseases,
but it worsen the effects in cyanotic congenital heart diseases. Increased nitric oxide
production seems to be the reason for the increased oxidative stress in cyanotic heart diseases,
which are worsened when exposed to laser irradiation.

DOI

10.21608/icmep.2008.29896

Authors

First Name

Ekram

Last Name

Abdel Salam

MiddleName

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Affiliation

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Email

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City

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Orcid

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First Name

Rabha

Last Name

El Shinawy

MiddleName

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Affiliation

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Email

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City

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Orcid

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First Name

Soheir

Last Name

Korraa

MiddleName

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Affiliation

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Email

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City

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Orcid

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First Name

Iman

Last Name

Abdel Meguid

MiddleName

E.

Affiliation

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Email

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City

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Orcid

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First Name

Gihan

Last Name

El Sharnouby

MiddleName

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Affiliation

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Email

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City

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Orcid

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Volume

4

Article Issue

International Conference on Mathematics and Engineering Physics (ICMEP-4)

Related Issue

5201

Issue Date

2008-05-01

Receive Date

2019-04-10

Publish Date

2008-05-01

Page Start

1

Page End

13

Print ISSN

2636-431X

Online ISSN

2636-4328

Link

https://icmep.journals.ekb.eg/article_29896.html

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https://icmep.journals.ekb.eg/service?article_code=29896

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18

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Original Article

Type Code

830

Publication Type

Journal

Publication Title

The International Conference on Mathematics and Engineering Physics

Publication Link

https://icmep.journals.ekb.eg/

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Article

Created At

22 Jan 2023