this study, Nile tilapia, Oreochromis niloticus (L.), (2 to 3g) was distributed into the four groups, control and three treated groups with copper sulfate at concentrations 5, 10 and 15 ml mol/L, according to design of experiment to four groups each group three replicate (control group) was contain 10 fish /100 L. and three treated groups with copper sulfate at concentrations 5, 10 and 15 ml mol/L for three months respectively. At the end of the experimental trial, blood samples were taken to determine the different physiological aspects. The growth parameters were affected by copper sulfate at concentrations 5, 10 and 15 ml mol/L. The high growth performance (WG. SGR and RGR) of tilapia, subject at control group and group exposed to copper sulfate at concentration 5 ml mol/L, respectively than the groups exposed to copper sulfate at concentration 10 and 15 ml mol/L, respectively. The best feed conversion ratio was obtained with control plus exposed to copper sulfate at concentration 5 ml mol/L, respectively. Also The highest values of protein efficiency ratio and protein productive were obtained with control plus exposed to copper sulfate at concentration 5 ml mol/L, respectively. The physiological aspects including erythrocyte count (RBCs), haemoglobin content (Hb) and haematocrit value (Hct) were significantly affected by exposed to copper sulfate at concentrations than control one. The overall results presented here indicate that the best growth performance of Nile tilapia was obtained at control fish.