This study closely inspected the effects of ovaprim, pituitary gland extract, and human chorionic gonadotropin on 11-ketotestosterone, and 17β-estradiol hormones. Females and males (T1, T2, and T3) were injected with Ova Prim as 0.5 ml/kg of fish, pituitary gland extract of catfish, and Human Chorionic Gonadotropin (HCG), respectively. Results showed that female gonadosomatic index of the spring season (10.45 ±0.35%) were significantly increased compared to other seasons: autumn followed by summer and winter seasons (0.82 ±0.06%). Results showed that T1 was better than T2, but T2 was better than T3 in all reproductive performance parameters. Plasma 17β-estradiol (ng/ml) in female catfish throughout 12 hours after injection of stimulating hormones ranged from 2.15 ng/ml in T1 to 1.65 ng/ml in T3 whereas testosterone in female of catfish throughout 12 hours were ranged from (0.031 ng/ml) in T1 to (0.036 ng/ml) in T3. Also, plasma 11-ketotestosterone (ng/ml) in male of catfish was recorded in T1 (0.154 ng/ml) followed by T2 (0.107 ng/ml) and lowest level in T3 (0.083 ng/ml) but plasma testosterone (ng/ml) in male was recorded in T1 (0.225 ng/ml) followed by T2 (0.189 ng/ml) and lowest level in T3 (0.143 ng/ml).