The activities of extracted jojoba meal were evaluated experimentally via using 240 Oreochromis niloticus that were distributed into 4 equal groups (each of sex replicates), Group l, Group 2, Group 3 and Group 4 fed on balanced diet supplemented with extracted jojoba meal by (0, 0.5, l and 2 g /kg) respectively. Hematological, Biochemical parameters, survival and disease resistance were determined . Blood samples were collected from the experimented fish , 30, 45 and 90 days from the time of the experiment to measure Red blood cell (RBCS) count, Hemoglobulin (Hb), Hematocrit (PCV) value, White blood cell (WBCS) count, Total protein, Albumin, Globulin, and A/G ratio. The protective effect of the jojoba meal was evaluated via challenge test using pathogenic A. hydrophila. Result relived that survival and disease resistance (%) were significantly increased in group supplemented with Extracted jojoba meal compared with control (Group l). The RBCS count, Hb, PCV value, WBCS count, Total protein, Globulin showed significant increase in all supplemented ( G 2,G3, G4) compared with control (G 1). On the other hand, a Significant decrease in Albumin value and A/G ratio has been observed in all supplemented groups (G2 ,G3,G4) compared with control. The challenge test showed increase level of protection in the all supplemented groups compared with control. These results revealed that supplemented with extracted jojoba meal could be a potential, provide increasing in hematology, biochemical parameters, and disease resistance of O. niloticus in aquaculture.