The present study was conducted to evaluate the effect of using zooplankton as a live food for Nile tilapia (Oreochromis niloticus), mullet (Mugil cephalus) and common carp (Cyprinus carpio) under monoculture and polyculture condition in concrete ponds on water quality, plankton abundance, growth, survival, stomach content and chemical composition of experimental fish. Twenty four concrete ponds were used at the Central Laboratory for Aquaculture Research (CLAR), Abbassa-Sharkia Governorate- Egypt. The experimental period extended for 140 days. The results showed that, water pH, nitrogen compounds (NH4, NO2 and NO3), dissolved oxygen and chlorophyll "a" significantly increased in ponds of artificial feed than those of zooplankton live food only. Using of zooplankton as a live food for fish improved the quality of fish. The water quality did not significantly (P>0.05) differ between monoculture and polyculture in the same feeding regimes. The results indicated also that, using zooplankton as a live food for fish species significantly (P<0.05) increased growth performance than those fed artificial feed. On the other hand, the growth performance of common carp has significantly (P<0.05) increased than Nile tilapia and mullet. Also, zooplankton was enough for fish to achieve suitable growth more than artificial feed. The detritus increased significantly in case of fish species fed with artificial feed, while it did not increase significantly in zooplankton live food treatments