This study was conducted at the intensive fish farm of Arab Fisheries Company (AFC) to investigate the effect ambient seasonal variations of three types of ground water quality (pure aquifer, recycled aquifer water and 50% mixture of both) on the reproduction of Oreochromis niloticus, Highly significant increase in seed production per pond and newly hatched fry per kilogram of females per season were obtained in spring within the pure aquifer water (8013.33±5.89 seed/pond, 5520.00±3.62 seed/ kg female/season respectively). Highly significant (p<0.05) differences were observed during the four seasons under ambient condition for the three types of water. The total fry production and/or relative fecundity of brooders were highly significant (p<0.05) 280041.25±40.3, 951.72±19.33 respectively under ambient condition of spring in pure aquifer water. In addition, highly significant differences (p<0.05) were found in water temperature among the studied four seasons. Although a wide variation in the ambient seasonal changes, the quality of water in reproduction pond were within the optimal range that required for reproduction of O. niloticus.