ManageChatDiscover
177074

Detection of Biocides Resistance Genes among Clinical Isolates of Both Methicillin-Resistant and Methicillin-Sensitive Strains of Staphylococcus Aureus

Article

Last updated: 04 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Basic Sciences

Abstract

BACKGROUND: Biocides compounds are widely used in infection control programs, including hand washing and skin decolonization before invasive procedures. However, its overuse leads to the emergence of Staphylococcus aureus with decreased biocides susceptibility, which became a problem in hospitals in different parts of the world.
AIM OF THE WORK: The present work aims to study the susceptibility of methicillin-resistant isolates of staphylococci aureus, obtained from different clinical samples to commonly used biocides, determines the prevalence of the biocide resistance genes, qacA/B, and qacC, among these isolates.
METHODS: Biocide's resistance genes [qacA/B, qacC] in Both Methicillin-Resistant and Methicillin-Sensitive Strains of Staphylococcus aureus were detected by PCR method. The minimum inhibitory concentrations [MICs] of Biocide agents were determined by broth dilution method, whereas antibiotic susceptibility was analyzed by disk diffusion and MICs methods according to Clinical and Laboratory Standards Institute [CLSI] criteria.
RESULTS: The frequency of Biocides resistance genes [qacA/B, qacC] was found to be high in our clinical staphylococci isolates. The qacA/B gene was detected in [63.3%] of MRSA isolates compared with only [23.3%] of MSSA isolates. Simultaneously, the qacC gene was detected in [63.3%] of MRSA isolates compared to only [13.3 %] of MSSA isolates., with the significant difference between both groups [p <0.05]. We also found an association between the presence of antiseptic resistance genes qacA/B, and increased MIC values to both savlon, hypochlorite, and Phenol [p <0.05]. We also showed that an association between the presence of antiseptic resistance genes qacC and the MICs to both savlon, hypochlorite, and Phenol [p <0.05].
CONCLUSION: Our study detects biocide resistance genes [qacA/B, qacC] in Staphylococcus aureus isolates in Egypt; more studies are needed to correct the biocide uses and to support infection control programs to avoid the development of new resistance mechanisms.  

DOI

10.21608/ijma.2021.62186.1260

Keywords

resistance, Biocides, Staphylococcus aureus, Methicillin-resistant, Biocides’ resistance genes

Authors

First Name

Walid

Last Name

Sabry

MiddleName

-

Affiliation

Department of Microbiology and Immunology, Damietta faculty of Medicine, Al-Azhar university, Egypt

Email

walid.sabry@domazhermedicine.edu.eg

City

Damiata

Orcid

-

First Name

Mohammed Yosry

Last Name

Al-Dakhakhni

MiddleName

Salah

Affiliation

Department Medical Microbiology and Immunology, Faculty of Medicine, Al-Azhar University, Egypt

Email

yosridakhakhni@gmail.com

City

Cairo

Orcid

-

First Name

Ibrahim

Last Name

Al-hoseiny

MiddleName

Mohammed

Affiliation

Department Medical Microbiology and Immunology, Faculty of Medicine, Al-Azhar University, Egypt

Email

ibrahim_m70@hotmail.com

City

Cairo

Orcid

-

First Name

Raafat Abd Rabbo

Last Name

Abdel Tawab

MiddleName

-

Affiliation

Department of Microbiology and Immunology, Damietta faculty of Medicine, Al-Azhar university, Egypt

Email

drraafat8@gmail.com

City

Menofia

Orcid

-

Volume

3

Article Issue

3

Related Issue

27050

Issue Date

2021-07-01

Receive Date

2021-02-08

Publish Date

2021-07-01

Page Start

1,602

Page End

1,610

Print ISSN

2636-4174

Online ISSN

2682-3780

Article File

IJMA_Volume 3_Issue 3_Pages 1602-1610.pdf

PDF . 1.5MB

Link

https://ijma.journals.ekb.eg/article_177074.html

Detail API

https://ijma.journals.ekb.eg/service?article_code=177074

Order

15

Type

Original Article

Type Code

816

Publication Type

Journal

Publication Title

International Journal of Medical Arts

Publication Link

https://ijma.journals.ekb.eg/

MainTitle

Detection of Biocides Resistance Genes among Clinical Isolates of Both Methicillin-Resistant and Methicillin-Sensitive Strains of Staphylococcus Aureus

Details

Type

Article

Created At

22 Jan 2023