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GATA3 and CK5/6 Immunohistochemical Expression in Urothelial Carcinoma: Diagnostic, Biological and Prognostic Significance

Article

Last updated: 04 Jan 2025

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-

Tags

Basic Sciences

Abstract

Background: Urothelial carcinomas [UCs] can be diagnostically challenging, particularly transurethral bladder tumors resection biopsies with limited material. Immunohistochemistry is a valuable instrument for diagnosis when morphology alone is inadequate. GATA-binding protein 3 [GATA3] and cytokeratin 5/6 [CK 5/6] contributes to the growth of UC. However, their correlative expression in UC and prognostic value has not been sufficiently investigated.   
The aim of the work: The current study aimed to evaluate the utility of GATA3 expression in UC and correlate that with CK 5/6 expression to verify different subtypes of UC and assess their prognostic significance.
Methodology: Here, we immunohistochemically stained GATA3 and CK 5/6 in 90 UCs samples by transurethral bladder tumor resection in a retrospective study, between May 2018 and February 2020. All were histopathologically evaluated and immunohistochemically stained for GATA3 and CK 5/6, and then correlated them with the clinicopathological parameters to investigate their clinical significance.
Results: GATA3 expression was seen in 76 patients [84.44 %]. There was significant correlation between GATA3 expression with the tumor histological grade and degree muscle invasion. There was a weak or even negative expression in high‑grade, invasive than the low‑grade, non-invasive tumor [P= <0.001]. CK5/6 was positive and focally positive in 27.78% with a significant correlation of CK5/6 expression with tumor grade and muscularis propria invasion. On the other side, tumors with diffuse GATA3 expression had low CK5/6 expression.
Conclusion: GATA3 and CK 5/6 should be used as sensitive and specific markers for UC. They can also be effectively used in the prediction of probable grade and tumor invasion in biopsied material with poor morphological characters; and thus, help in the future appropriate treatment.

DOI

10.21608/ijma.2021.67390.1280

Keywords

Urothelial carcinoma, Transurethral bladder tumor resection, Muscle-invasive bladder cancers, GATA‑3, CK 5/6, Immunohistochemical

Authors

First Name

Marwa Abdel Moneim

Last Name

El Kholy

MiddleName

-

Affiliation

Department of Pathology, Faculty of Medicine for Girls, Al-Azhar University, Egypt

Email

marwapatho@yahoo.com

City

Cairo

Orcid

-

First Name

Mohamed

Last Name

Ali

MiddleName

Yousef

Affiliation

Department of Pathology, Faculty of Medicine, Al-Azhar University, Egypt.

Email

mohamedyousef79@gmail.com

City

Cairo

Orcid

-

First Name

Ahmed

Last Name

Aboelsaad

MiddleName

Yousef

Affiliation

Department of Urology, Damietta Faculty of Medicine, Al-Azhar University, Egypt

Email

aboelsaadurology@hotmail.com

City

Man

Orcid

0000-0001-9851-8120

First Name

Ahmed

Last Name

Abdel Gawad

MiddleName

M.

Affiliation

Department of Urology, Damietta Faculty of Medicine, Al-Azhar University, Egypt.

Email

aa.urology@domazhermedicine.edu.eg

City

Talkha

Orcid

0000-0001-7051-6019

Volume

3

Article Issue

2

Related Issue

24821

Issue Date

2021-04-01

Receive Date

2021-03-11

Publish Date

2021-04-01

Page Start

1,306

Page End

1,315

Print ISSN

2636-4174

Online ISSN

2682-3780

Article File

IJMA_Volume 3_Issue 2_Pages 1306-1315.pdf

PDF . 1.6MB

Link

https://ijma.journals.ekb.eg/article_165417.html

Detail API

https://ijma.journals.ekb.eg/service?article_code=165417

Order

19

Type

Original Article

Type Code

816

Publication Type

Journal

Publication Title

International Journal of Medical Arts

Publication Link

https://ijma.journals.ekb.eg/

MainTitle

GATA3 and CK5/6 Immunohistochemical Expression in Urothelial Carcinoma: Diagnostic, Biological and Prognostic Significance

Details

Type

Article

Created At

22 Jan 2023