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150867

Efficacy of Nitazoxanide Alone or Loaded with Silica Nanoparticle for Treatment of Cryptosporidiosis in Immunocompetent Hosts

Article

Last updated: 04 Jan 2025

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Subjects

-

Tags

Basic Sciences

Abstract

Background: Cryptosporidiosis is a major health problem for humans and animals with severe consequences in immune deficient hosts. There is no effective approved drug therapy against Cryptosporidium till now and it is increasingly necessary for evaluating new potential drugs. Nanoparticles are promising for effective treatment of parasitic diseases, as an emerging drug carriers.
The aim of the work: Studying nitazoxanide efficacy alone and compared to nitazoxanide loaded with silica nanoparticles in the treatment of cryptosporidiosis in immunocompetent mice infected with Cryptosporidium.
Materials and methods: The study included 50 Swiss albino mice subdivided into five subgroups, including treatment either with silica nanoparticles alone, with Nitazoxanide alone or by Nitazoxanide loaded with silica nanoparticles. We included infected non-treated and non- infected non treated mice in the study as the positive and the negative controls, respectively. The post-treatment evaluation at two and three weeks was done using parasitological stool examination, histological examination of the intestine and liver, and serological screening for anti-cryptosporidium IgG and IgM using ELISA at the 3rd week only.
Results: Decreased percentages of cryptosporidium oocyst passage in all treated immunocompetent mice groups and an improvement on the intestinal and liver histopathology observed after two weeks and a significant oocyst count reduction and near total histopathological cure observed after the third week with superior results observed in groups treated with Nitazoxanide loaded silica.
Conclusion: Cryptosporidium infection is a potentially harmful condition. Major histopathological changes and clinical deterioration occur in immunocompromized hosts requiring more available therapeutic options. The nitazoxanide loaded silica showed promising results in the treated groups superior to nitazoxanide alone. This gives promising results encouraging further evaluation studies.

DOI

10.21608/ijma.2021.55788.1237

Keywords

Cryptosporidium, Nitazoxanide, silica, Nanoparticles, Parasitic diarrhea

Authors

First Name

Alaa

Last Name

Metawae

MiddleName

Gamal

Affiliation

Department of Parasitology, Damietta Faculty of Medicine, Al-Azhar University, Egypt

Email

dr_alaagamal@yahoo.com

City

-

Orcid

-

First Name

Ahmed

Last Name

Bayoumy

MiddleName

M.

Affiliation

Department of Parasitology, Faculty of Medicine, Al-Azhar University, Egypt

Email

bayoumy45@yahoo.com

City

-

Orcid

-

First Name

Ibrahim

Last Name

Ali

MiddleName

Rabee

Affiliation

Department of Immunology and Evaluation of Drugs, Theodor Bilharz Research Institute, Egypt

Email

ibrahimshalash@yahoo.com

City

-

Orcid

-

First Name

Olfat

Last Name

Hammam

MiddleName

A.

Affiliation

Department of Pathology, Theodor Bilharz Research Institute, Egypt

Email

totoali1@hotmail.com

City

-

Orcid

-

First Name

Khaled A. Temsah

Last Name

A. Temsah

MiddleName

-

Affiliation

Department of Parasitology, Damietta Faculty of Medicine, Al-Azhar University, Egypt

Email

drkhaled2008@yahoo.com

City

Gharbia

Orcid

-

Volume

3

Article Issue

2

Related Issue

24821

Issue Date

2021-04-01

Receive Date

2020-12-29

Publish Date

2021-04-01

Page Start

1,229

Page End

1,239

Print ISSN

2636-4174

Online ISSN

2682-3780

Article File

IJMA_Volume 3_Issue 2_Pages 1229-1239.pdf

PDF . 2.2MB

Link

https://ijma.journals.ekb.eg/article_150867.html

Detail API

https://ijma.journals.ekb.eg/service?article_code=150867

Order

10

Type

Original Article

Type Code

816

Publication Type

Journal

Publication Title

International Journal of Medical Arts

Publication Link

https://ijma.journals.ekb.eg/

MainTitle

Efficacy of Nitazoxanide Alone or Loaded with Silica Nanoparticle for Treatment of Cryptosporidiosis in Immunocompetent Hosts

Details

Type

Article

Created At

22 Jan 2023