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95155

Polymerase Chain Reaction versus Slit Skin Smear in Diagnosis of Leprosy; A Cross Sectional Study

Article

Last updated: 22 Jan 2023

OverviewSimilar Items

Subjects

-

Tags

Dermatology

Abstract

Background: Leprosy is a chronic infectious disease where delay in diagnosis or treatment can lead to deformities and disabilities for the rest of the patient's life. Definite diagnosis of leprosy has long been based on clinical picture, histopathology and/or the detection of acid-fast bacilli (AFB) from tissue smears or tissue sections stained by Ziehl-Neelsen. 
Aim of the work: We evaluatedin this study, the usefulness of PCR for detection of M.leprae in the serum, as less invasive technique, in comparison to skin slit smear  for the diagnosis of  leprosy.
Patients and Methods:This study included 30 non-treated patients of leprosy (9 multibacillary, 21 paucibacillary) skin slit smears had been taken. In addition, serum samples had been collected for polymerase chain reaction [PCR] test.
Results: Negative Slit Skin Smear for AFB had been obtained in 20 cases with a rate of positivity of 33%. Serum PCR was positive in 20 patients giving an overall rate of positivity of 67% which confirm the diagnosis in 11 out of 20 cases with slit skin smear [SSS] negative.
Conclusion: PCR does show to be more sensitive than slit skin smear [SSS] indicating its future use for diagnostic purposes especially in early leprosy cases. 

DOI

10.21608/ijma.2020.30181.1126

Keywords

Leprosy, polymerase chain reaction, Slit Skin Smear, Mycobacterium Leprae, Acid-Fast Bacilli

Authors

First Name

Nehal

Last Name

Mostafa kamel

MiddleName

Magdy

Affiliation

Department of Dermatology, elsenbellawin General Hospital, Ministry of Health and Populations, Egypt

Email

nehalmagdy1985@gmail.com

City

Mansoura

Orcid

-

First Name

Osama

Last Name

Hashem

MiddleName

-

Affiliation

Department of Dermatology, Damietta Faculty of Medicine, Al-Azhar University, Egypt

Email

osama.os129@domazhermedicine.edu.eg

City

-

Orcid

0000000337730990

First Name

Hassan

Last Name

Khodair

MiddleName

Mohamed

Affiliation

Dermatology Department, Damietta Faculty of Medicine, Al-Azhar University, Egypt.

Email

drhassankhodair@gmail.com

City

-

Orcid

-

First Name

Hesham

Last Name

Abd El-Samee

MiddleName

Samir

Affiliation

Department of Clinical Pathology, Damietta Faculty of Medicine, Al-Azhar University, Egypt.

Email

hsamir87@domazhermedicine.edu.eg

City

-

Orcid

-

Volume

2

Article Issue

3

Related Issue

16043

Issue Date

2020-07-01

Receive Date

2020-05-13

Publish Date

2020-07-01

Page Start

650

Page End

654

Print ISSN

2636-4174

Online ISSN

2682-3780

Article File

IJMA_Volume 2_Issue 3_Pages 650-654.pdf

PDF . 261.9kB

Link

https://ijma.journals.ekb.eg/article_95155.html

Detail API

https://ijma.journals.ekb.eg/service?article_code=95155

Order

24

Type

Original Article

Type Code

816

Publication Type

Journal

Publication Title

International Journal of Medical Arts

Publication Link

https://ijma.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023