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Histological and Immunohistochemical Studies on The Role of Stem Cells on The Burned Skin of Adult Male Albino Rats

Article

Last updated: 04 Jan 2025

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-

Tags

Basic Sciences

Abstract

Background: Mesenchymal stem cells have delivered new approaches to the management of burn healing in severe skin injuries.
Aim of the work: To evaluate the effect of bone marrow-derived mesenchymal stem cells (BMSCs) on healing of induced deep 2nd degree skin burns in albino rats.
Methodology: 55 male albino rats weighed 200gm were divided into 5 groups, 5 animals in group I (control) and group II (burn model), 15 animals in group III (untreated), group IV and group V (MSCs treated). Groups III, IV and V were subdivided into 3 subgroups that were sacrificed after 7, 14 and 21 days respectively. Deep 2nd degree burn wounds were induced on a 4 cm2 area on the back of rats in groups II, III, IV and V by brass probes stabilized at 88–90°C and contacted to the shaved dorsal skin of rats for 20 seconds without exerting any external pressure. Mesenchymal stem cells were isolated from the bone marrow of 5 young rats, weighed 100gm then intra-dermally and intraperitoneally injected.
Results: By the end of the third week, the wounds of BMSCs-treated groups showed full regeneration of epidermis, re-organization of collagen and decrease in VEGF immunopositive cells. Delayed wound healing was seen in 20% of systemically treated rats. Significant increase in the mean area percent of collagen fibers was detected in topically treated group.
Conclusion: Both methods of BMSCs injection were effective in healing of full thickness skin wound but topical method was more effective.

DOI

10.21608/ijma.2020.20386.1050

Keywords

stem cells, skin, vascular endothelial growth factor, Wound healing, scar

Authors

First Name

Fatma

Last Name

Abd Allah

MiddleName

M.

Affiliation

Department of Histology, Damietta Faculty of Medicine, Al-Azhar University, Egypt.

Email

fatma.mamhisto@domazhermedicine.edu.eg

City

-

Orcid

-

First Name

Ashraf

Last Name

Moustafa

MiddleName

M

Affiliation

Department of Histology, Faculty of Medicine, Al-Azhar University

Email

dr.osama784@azhar.edu.eg

City

-

Orcid

-

First Name

Lotfy

Last Name

Moahmmed

MiddleName

S

Affiliation

Department of Histology, Faculty of Medicine, Al-Azhar University

Email

dr.lotfy.s@gamil.com

City

-

Orcid

-

First Name

Ezz El-Dein

Last Name

Abd Allah

MiddleName

E

Affiliation

Department of Histology, Damietta Faculty of Medicine, Al-Azhar University, egypt

Email

ezz.abdallah@gmail.com

City

-

Orcid

-

Volume

2

Article Issue

1

Related Issue

10770

Issue Date

2020-01-01

Receive Date

2019-12-01

Publish Date

2020-01-01

Page Start

265

Page End

291

Print ISSN

2636-4174

Online ISSN

2682-3780

Article File

IJMA_Volume 2_Issue 1_Pages 265-291.pdf

PDF . 1.5MB

Link

https://ijma.journals.ekb.eg/article_69613.html

Detail API

https://ijma.journals.ekb.eg/service?article_code=69613

Order

18

Type

Original Article

Type Code

816

Publication Type

Journal

Publication Title

International Journal of Medical Arts

Publication Link

https://ijma.journals.ekb.eg/

MainTitle

Histological and Immunohistochemical Studies on The Role of Stem Cells on The Burned Skin of Adult Male Albino Rats

Details

Type

Article

Created At

22 Jan 2023