Abstract
Bovine tuberculosis (bTB) is a chronic disease caused by Mycobacterium bovis which cause high morbidity and mortality in animals and human. The objective of this study is to investigate the prevalence of tuberculosis in bovine slaughtered animals and its prevalence in human patients in Gharbia governorate, Egypt. A total of 600 random animals (300 cows, 150 calves, and 150 buffaloes) were routinely examined in one of slaughterhouses of Gharbia governorate, Egypt during the year 2021 for detection of bovine tuberculosis. The suspected tuberculous lesions were collected from infected cattle from different organs the bacteriological examination revealed that the isolation rate was 70%, 80%, 66.7% in cattle, buffaloes and calves, respectively. PCR technique was used to confirm the results of bacteriological examination in 17 positive TB samples in buffaloes and bovine calves. The results showed that 94.1% agreement between bacteriological examination and PCR technique, as well as 35 sputum samples (23 samples from males and 12 females) were collected from suspected patients in the same governorate. The results also showed that 25.7% of patients were positive for TB with a higher prevalence in males (20%) than females (5.7%). PCR technique was used to confirm the results of bacteriological culture on 9 positive human being samples. The results showed that bacteriological culture was 100% consistent with PCR testing for human samples. So, the research documented the occurrence of bovine tuberculosis in slaughtered animals and human in Gharbia governorate which needs to reevaluate the bTB eradication program.