Antimicrobial resistance to antibiotics is a major barrier in treating serious nosocomial infections. There is studies evaluating the resistance profile of the Y. enterocolitica strains are obtained from various countries. Therefore, this study aims evaluating the 31 Y. enterocolitica isolates in terms of their antimicrobial resistance. They were previously isolated by the same authors from 225 random samples of cow milk, beef, chicken meat, diarrheic cow feces, and diarrheic human stool of patients suffering from vomiting and diarrhea that were collected from different shops, dairy herds, and hospitals (45 for each), at Kaliobia Governorate of Egypt, besides detection of some antibiotics resistant genes in some strains. This study cleared that the isolates were extremely resisting oxacillin, then tetracycline, Nalidixic acid, and cefoxitin. Meanwhile, they were extremely sensitive to norfloxacin, then ciprofloxacin, cefotaxime, and gentamycin. Moreover, PCR appeared that aadA1, tetA(A), qnrA, and blaCTX-M genes were detected in all eight studied Y. enterocolitica isolates. So, the study concluded that the antimicrobial-resistant Y. enterocolitica strains in animal-origin foods could be a public health concern for consumers. They could also be considered for antimicrobial resistance control and food safety measures.