The present study is directed to isolate and identify pathogenic E. coli from urine of humans and pet animals (dogs and cats) suffering from UTIs who admitted to different hospitals and veterinary clinics (35 for each), at Kaliobia Governorate and to determine their antimicrobial sensitivity, beside detection of some resistance genes. The results revealed that, that E-coli were isolated from 41 from the examined samples where , (15/42.9%) from human; (14/40.0%) and (12/34.3 %) from dogs and cats urine samples
,respectively . The isolated E. coli were highly sensitive to meropenem followed by nitrofurantoin; norfloxacin and gentamycin. Meanwhile, they were highly resistant to nalidixic acid followed by ampicillin; tetracycline; cefotaxime; azithromycin; co- trimoxazole; streptomycin and ciprofloxacin .Moreover, PCR results showed that, qnrS; aadA1 and blaTEM antibiotic resistant genes were amplified in all sex studied E. coli strains and mphA resistant gene was amplified in five studied strains .Therefor, the study concluded that, uropathogenic E. coli of humans, dogs and cats affected with UTIs may be the most important pathogen with multiple antibiotic resistances and there were positive correlation between the presence of qnrS; mphA; aadA1 and blaTEM genes in these strains with the phenotypic resistance to the antibiotics of these groups.