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273798

Detection of FSHR gene variants associated with sperm motility in Egyptian buffalo bulls

Article

Last updated: 04 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Animal Production

Abstract

This study's goal was to use the DNA sequence techniques to find new mutations in the follicle-stimulating hormone receptor exon 10 (FSHR) in Egyptian buffalo bulls with different sperm motility. A total of 50 Egyptian buffalo bulls aged 3–4 years old, at Kafr El-Sheikh Governorate (breeding station of Mahallet Mousa), Egypt were investigated. The bulls were divided into two groups according to spermatozoa motility: the first group of high sperm motility (> 60%, n = 32) and the second group of low sperm motility (< 60%, n = 18). Blood samples were collected to obtain genomic deoxyribonucleic acid, and to perform polymerase chain reaction with an annealing temperature of 57 °C to amplify 306 bp of the FSHR exon 10 gene. The results showed that all the investigated buffalo bulls were monomorphic and had the same genotype. We may conclude that there no different variants of FSHR gene associated with different sperm motility, therefore we need to study more genes in relation to sperm motility.

DOI

10.21608/bvmj.2022.143994.1532

Keywords

Egyptian buffalo, Bull, semen quality, FSHR gene

Authors

First Name

Eman

Last Name

Sallam

MiddleName

Ahmad

Affiliation

Animal and Poultry Production, Department of Animal Wealth Development, Faculty of Veterinary Medicine, Benha University, Benha 13736, Egypt

Email

eman.salam@fvtm.bu.edu.eg

City

-

Orcid

0000-0001-7017-6077.

First Name

Liza

Last Name

Mohammed

MiddleName

-

Affiliation

animal wealth development department, faculty of veterinary medicine, benha univeristy

Email

liza.reda@fvtm.bu.edu.eg

City

-

Orcid

0000-0003-1172-0823

First Name

Samy

Last Name

Darwish

MiddleName

-

Affiliation

Department of Animal Biotechnology, Animal production research institute, Agriculture research center, Egypt

Email

samydarwish2030@yahoo.com

City

-

Orcid

-

First Name

mohammed

Last Name

Hegazy

MiddleName

-

Affiliation

Department of Buffalo Breeding, Animal production research institute, Agriculture research center, Egypt.

Email

mmgad2120@gmail.com

City

-

Orcid

-

First Name

Al- shimaa

Last Name

El-Naby

MiddleName

-

Affiliation

5Department of Theriogenology, Faculty of veterinary medicine Benha University, Egypt

Email

alshimaa.assabelnaby@fvtm.bu.edu.eg

City

-

Orcid

-

First Name

Aya

Last Name

Azzam

MiddleName

-

Affiliation

6Animal hygiene and veterinary Management Department, Faculty of veterinary medicine Benha University,, Egypt

Email

aya.azzam@fvtm.bu.edu.eg

City

-

Orcid

0000-0002-1988-9933

Volume

42

Article Issue

2

Related Issue

37149

Issue Date

2022-10-01

Receive Date

2022-06-13

Publish Date

2022-10-01

Page Start

191

Page End

193

Print ISSN

1110-6581

Article File

BVMJ_Volume 42_Issue 2_Pages 191-193.pdf

PDF . 847.7kB

Link

https://bvmj.journals.ekb.eg/article_273798.html

Detail API

https://bvmj.journals.ekb.eg/service?article_code=273798

Order

273,798

Type

Original Article

Type Code

812

Publication Type

Journal

Publication Title

Benha Veterinary Medical Journal

Publication Link

https://bvmj.journals.ekb.eg/

MainTitle

Detection of FSHR gene variants associated with sperm motility in Egyptian buffalo bulls

Details

Type

Article

Created At

22 Jan 2023