Infectious bovine rhinotracheitis (IBR), is a contagious disease in cattle. BoHV-1 is one of the most common viral infections in cattle throughout the world. It's highly contagious and most commonly found in cattle herds. During the year 2021, a cross-sectional study was conducted to assess seroprevalence and risk factors related to bovine herpesvirus-1. A total of 300 serum samples were collected from cattle raising in three governorates in the Nile Delta region of Egypt and examined using a commercial ELISA kit. The seroprevalence rate of BoHV-1 infection was 30.67%. Multivariable logistic regression analysis revealed that the BoHV-1 infection is strongly associated with the species of animal, age, contact with small ruminants, and herd size. However, locality, sex, and purpose of herd showed non significant effect of prevalence of infection. This study confirmed the presence of BoHV-1 in Egypt as an endemic disease in dairy and fattening herds and the application of an efficient control program is necessary to control the disease.