The aim of this study was to describe the ultrasonographic, anatomical and histological features of superficial lymph nodes (LN) (mandibular, superficial cervical, subiliac, scrotal and supramammary) in donkeys. Twenty-four donkeys (3-6 years old and 120-180 kg BW) were examined ultrasonographically for characterization of LN appearance and echogenicity. LNs were dissected and processed for histological examination. Our examination revealed that mandibular LN located between mandibular rami along each side of omohyoid muscles and ultrasonographically appeared as long lobulated structure with clear anechoic lobules and hyperechoic interlobular trabeculae. Superficial cervical LN was sited at 5 cm dorsal to the shoulder joint at the caudal end of the jugular vein and ultrasonographically appeared as a quadrilateral to oval multi-lobulated structure with hypoechoic lobules interspersed with fine interlobular echogenic trabeculae and delineated by a thick distinct echogenic capsule. Subiliac LN located at midway between patella and coxal tuber and ultrasonographically appeared as an oval wholly anechoic structure with a fine central hypoechoic septum and delineated by a thick hyperechoic capsule. Scrotal LN located at the level of the superficial inguinal ring either cranial or caudal to the spermatic cord and ultrasonographically appeared as a small oval to round shape structure with an entire anechoic content. Supramammary LN localized on the lateral border of udder base at the superficial inguinal ring and ultrasonographically appeared as a rectangular to ovoid structure with an entire anechoic content. In conclusion, the present data set a preliminary reference for the normal ultrasonographic anatomy of adult donkeys superficial LNs.