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268435

Phylogenetic analysis of VP1 foot and mouth disease virus strains circulating between 2018-2020, Egypt

Article

Last updated: 04 Jan 2025

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Subjects

-

Tags

Microbiology

Abstract

Foot-and-mouth disease (FMD) is posing serious economic losses if not stringently controlled. This study aimed to characterize FMD virus (FMDV) in suspected samples which were collected from cattle and buffalo in Egypt between 2018 and 2020. Pan-serotype FMDV real time reverse transcription PCR (RT-PCR) assay that targets 3D genes of the FMDV were used to the preliminary laboratory diagnosis. The study was also performed with virus isolation on BHK-21 cells, conventional RT-PCR, and sequencing. FMDV serotypes A, O, and SAT2 were detected in all the examined 44 samples. Phylogenetic analysis of VP1 sequence products showed that recent FMDV outbreaks that occurred during 2018, 2019, and 2020 in Egypt were caused by serotypes SAT2 of Lib-12 lineage. Serotype O of East Africa 3 (EA-3) was detected in 2019 samples while serotype A of African topotype/G-IV was detected in 2020. The study findings recommended the significance of booster sampling of representative FMDV from the bordering countries as such protocol might be impacted on FMD control strategy as a transboundary viral disease.

DOI

10.21608/bvmj.2022.114191.1492

Keywords

FMDV, PCR, 3D gene, Phylogenetic analyses, VP1 gene

Authors

First Name

Ayah

Last Name

Hassan

MiddleName

-

Affiliation

Genome Research Unit, Animal Health Research Institute, Agricultural Research Center, 12618 Giza, Egypt

Email

ayahmhassan@ahri.gov.eg

City

Giza

Orcid

0000-0003-4202-6025

First Name

zeinab

Last Name

aboezz

MiddleName

R.A.

Affiliation

Virology department, faculty of vet medicine, Benha university.

Email

zeinab.aboezz@fvtm.bu.edu.eg

City

-

Orcid

-

First Name

Ayman

Last Name

El-Habbaa

MiddleName

Said

Affiliation

Virology Department, Faculty of Veterinary Medicine, Benha University, Moshtohour, Kalubeya

Email

ayman.alhbbak@fvtm.bu.edu.eg

City

Moshtohour, Kalubeya

Orcid

0000-0003-3514-3573

First Name

Momtaz

Last Name

Shahein

MiddleName

-

Affiliation

Virology Department, Animal Health Research Institute, Agricultural Research Center, 12618 Giza, Egypt

Email

momtaz.shahein@yahoo.com

City

-

Orcid

-

First Name

Naglaa

Last Name

Hagag

MiddleName

-

Affiliation

Genome Research Unit, Animal Health Research Institute, Agricultural Research Center, 12618 Giza, Egypt

Email

naglaahagagahri@gmail.com

City

-

Orcid

0000-0002-2598-3264

First Name

Saad

Last Name

Sharawy

MiddleName

-

Affiliation

Faculty of Veterinary Medicine, Benha University

Email

saad.sharawy@fvtm.bu.edu.eg

City

Benha University

Orcid

0000-0003-3647-2440

Volume

42

Article Issue

2

Related Issue

37149

Issue Date

2022-09-01

Receive Date

2022-01-16

Publish Date

2022-09-01

Page Start

7

Page End

13

Print ISSN

1110-6581

Online ISSN

2974-4806

Article File

BVMJ_Volume 42_Issue 2_Pages 7-13.pdf

PDF . 1.8MB

Link

https://bvmj.journals.ekb.eg/article_268435.html

Detail API

https://bvmj.journals.ekb.eg/service?article_code=268435

Order

2

Type

Original Article

Type Code

812

Publication Type

Journal

Publication Title

Benha Veterinary Medical Journal

Publication Link

https://bvmj.journals.ekb.eg/

MainTitle

Phylogenetic analysis of VP1 foot and mouth disease virus strains circulating between 2018-2020, Egypt

Details

Type

Article

Created At

22 Jan 2023