There are challenges when monitoring peri-ovulatory ovarian activity in domestic bitches which only ovulate once or twice a year. There is difficulty to distinguish between pre-ovulatory follicles and/or corpus luteum. As luteinization of follicles subsists before ovulation, follicles do not collapse after ovulation. Thus, the aim of the present study was to predict the exact day of ovulation. Twenty-seven bitches of mixed breeds were examined daily from onset of proestrus to five days post ovulation by vaginal smear, real-time B-mode and Doppler ultrasonography. Ultrasound was conducted in both standing and lateral recumbency positions using microconvex and linear probes. Vaginal swabs were stained by DIFF QUICK stain. There was high cornification rate (90 %) of vaginal epithelium and substantial increase in follicular size (0.55±0.02 cm) at ovulation day. There was significant increase in ovarian blood perfusion at Day 0 and 1. Maximum coloring seen two days around ovulation time. We concluded that color Doppler ultrasound performed once daily was more accurate in predicting the preovulatory LH peak than B-mode ultrasound and vaginal cytology..