The current study was carried out to investigate the protective effect of conjugated linoleic acid against doxorubicin-induced toxicity in rats. For this purpose, 40 rats were divided into four groups. control group: rats were injected with a sterile distilled water. Doxorubicin group: rats were injected IP with doxorubicin. Conjugated linoleic acid ( CLA) group: rats were received CLA orally. Doxorubicin +CLA group: rats were injected with doxorubicin for 2 weeks then received CLA once daily for 4 weeks. Collection of serum and spleen samples were performed at 4th and 6th week of experiment. Serum was used for evaluation of immunological markers, protein electrophoresis, kidney functions, liver enzymes.Tissue specimens from spleen collected for histopathology examination. The results revealed that there was significant decreases in IgE and IgM, while significant increases in IL-6, urea, creatinine, ALT, AST, ALP were recorded in doxorubicin group. Moreover, histological examination of splenic tissues of doxorubicin group showed congestion of red pulp,lymphoid depletion of white pulp and severe necrosis of lymphoid follicle. Treatment with CLA showed improvement in aforementioned parameters in the first and second check points. It could be concluded that CLA has protective effect against immunosuppression induced by doxorubicin.