In the present study, iron oxide (FeO) and zinc oxide (ZnO) nanoparticles were studied for their potential effect against Fasciola spp. in vitro. Fasciola adult worms were incubated for 24 h with three concentrations (0.004, 0.008 and 0.012% w/v) for both FeO and ZnO. The parasites motility and mortality were recorded at different times. The result showed that both nanoparticles could significantly reduce the worms' motility and increased their mortality compared to the control group. Moreover, the parasites were examined by high-resolution SEM which showed an upper body dislocation with swelling of the worm's body, roughness, hemorrhage, desquamation and complete loss of the spine induced by 0.012% conc. of both FeO and ZnO nanoparticles was more than other concentrations. It can be concluded that FeO and ZnO nanoparticles had potent fasciocidal activity especially at 0.012% Conc of both FeO and ZnO nanoparticles.
Key words: Fasciola spp, infection, FeO, ZnO, Nanoparticle, SEM, Dakhla Oasis