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208245

A Recent Non cytopathogenic bovine viral diarrhea virus detected by immunofluorescence and reverse transcriptase polymerase chain reaction

Article

Last updated: 04 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Microbiology

Abstract

In the present study, a non-cytopathogenic bovine viral diarrhea virus (BVDV) field strains from bovine semen samples were detected by immunofluorescence (IF) and reverse transcriptase polymerase chain reaction (RT-PCR). Two out of five semen samples tested positive for the presence of BVDV antigen employing antigen capture ELISA kits. The two semen samples were injected in Madin-Darby bovine kidney (MDBK) cells in a trial for BVDV isolation. The chosen two samples were not cytopathic and subjected for further identification and genotyping using one step reverse transcriptase polymerase chain reaction (RT-PCR) assay. The isolated BVDV field strains from semen were found to be BVDV genotype 1.

DOI

10.21608/bvmj.2021.79012.1427

Keywords

Noncytopathic BVDV, Semen, MDBK, RT-PCR, Genotype1

Authors

First Name

Samia

Last Name

Abdelkareem

MiddleName

-

Affiliation

Viral Diseases Research Unit, Animal Reproduction Research Institute (ARRI), Agricultural Research Center (ARC), Giza, Egypt

Email

samiaelnagar.21@gmail.com

City

6 th october elGiza

Orcid

-

First Name

Ayman

Last Name

El-Habbaa

MiddleName

Said

Affiliation

Virology department, faculty of veterinary medicine, Benha university, Moshtohor, Egypt

Email

aymanelhabbak@gmail.com

City

Moshtohor

Orcid

0000-0003-3514-3573

First Name

zeinab

Last Name

aboezz

MiddleName

R.A.

Affiliation

Virology department, faculty of vet medicine, Benha university.

Email

zeinab.aboezz@fvtm.bu.edu.eg

City

-

Orcid

-

First Name

Ehab

Last Name

ElNahas

MiddleName

-

Affiliation

Virology Department, Faculty of Veterinary Medicine, Benha University, Moshtohor 13736, Kalubia, Egypt

Email

ehab.nasar@fvtm.bu.edu.eg

City

Toukh

Orcid

-

Volume

41

Article Issue

1

Related Issue

28214

Issue Date

2021-10-01

Receive Date

2021-06-05

Publish Date

2021-10-01

Page Start

39

Page End

41

Print ISSN

1110-6581

Article File

BVMJ_Volume 41_Issue 1_Pages 39-41.pdf

PDF . 353.5kB

Link

https://bvmj.journals.ekb.eg/article_208245.html

Detail API

https://bvmj.journals.ekb.eg/service?article_code=208245

Order

8

Type

Original Article

Type Code

812

Publication Type

Journal

Publication Title

Benha Veterinary Medical Journal

Publication Link

https://bvmj.journals.ekb.eg/

MainTitle

A Recent Non cytopathogenic bovine viral diarrhea virus detected by immunofluorescence and reverse transcriptase polymerase chain reaction

Details

Type

Article

Created At

22 Jan 2023