Platelet rich plasma (PRP) and platelet rich fibrin (PRF) are considered excellent concentrated sources of growth factors (PDGF, TGF-β, VEGF, bFGF) and cytokines fundamental for wound healing. The aims of this study were to evaluate and compare the effect of PRP and PRF on the second intention wound healing through clinical vision, histopathological examination, and changes in the IL10 and TGF-β genes expression. Fifteen adult dogs were used in this study; induction of a 3cm diameter total thickness cutaneous injury at the right chest region. The animals were divided into three groups equally, and the wounds were treated twice weekly for three successive weeks. The first group (Group A) received only normal saline (control group). The second group was treated by PRP (group B). The third group received PRF treatment (group C). Clinical Evaluation, Molecular studies of interlukin 10 (IL10) and transforming growth factor-β (TGF-β) gene expression, and histopathological examination were used to demonstrate the difference between the three treatment regimens. Results showed a non-significant negative correlation between weight loss and WHR% and showed a significantly high positive correlation between treatment cost either by PRP or PRF with IL10 (0.79*) and WHR% (wound healing rate) (0.994**). The IL10 significantly increased in PRP group, while TGF-b was significantly increased in the PRF group. This study concluded that the PRP and PRF exhibited higher regeneration capacity and accelerates the quality of wound healing