This work was carried out to evaluation the Reno-protective effect and anti-inflammatory activity of rutin in guinea pigs. Twenty four male guinea pigs categorized into 4 groups, six pigs in each group. Group one as a normal control given (2ml/kg)distilled water. Second group as non-treated control were given 5% of carboxymethyl cellulose orally. Third group, animals were received Rutin (20mg/kg). The fourth group were given aqueous suspensions of Indomethacin (10mg/kg) in 5% Polysorbate 80 orally once daily for seven days. For model of chronic inflammation each pig were anesthetized by light ether and then after thirty minutes a sterile cotton pellet weighing (50 mg±1) was implanted under the skin on both sides for the scapular region. All pigs were anaesthetized by using chloroform on the 8th day then the blood collected. They were dissected kidney and put it in 10% formalin for histological test. Then isolated the cotton pellets from each, dried at 60Co and estimated the dry weight. Administration of rutin (20mg/kg body weight) for seven days showed significant(P<0.05) inflammation inhibition which percent of inhibition were 33% for(Rutin), 37% for (Indomethacin) and 0% for control. Also, Rutin (20 mg/kg) improved the biochemical related to inflammation. This was also supported by histopathological findings of the examined kidneys. On conclusion, Rutin possess anti-inflammatory activity and protective role against granuloma-induced alterations.