Equine influenza virus (EIV), is of the important commen and important respiratory infectious diseases of horses. Vaccination can reduce the the prevalence and severity of disease. In the current study, a trial to improve the strength and duration of immune response through using of montanide oil ISA 206, saponin and Mycobacterium pheli extract as adjuvants for an egg grown inactivated EIV [A/equine/Alexandria/1/08 (H3N8)]. Safety and potency of the prepared vaccine studied by inoculation into guinea pigs and horses then evaluated using Haemagglutination inhibition (HI) test for humeral immune response. Cellular immune response evaluated in horses inoculated with inactivated EIVvaccine adjuvanted with Mycobacterium pheli extract using lymphocyte blastogenesis. The vaccines were safe for g.pigs and horses which inducing HI antibodies titer that persist for 10 months post-inoculation in vaccinated horses. EI vaccine adjuvanted with montanide oil ISA 206 was more potent than the other vaccines adjuvanted either with saponin or M.pheli extract. EI vaccine adjuvanted with montanide oil ISA 206 also keeps at 4oC for over one year and at room temperature for 6 months without effect on its immunogenic characters.