The current study designed to evaluate the role of pluripotent stem cells (iPSc) on experimentally-induced ovarian ablation (OA) by evaluating of hematological parameters, FSH and E2 changes, molecular and histopathological examination. 50 female rats were divided into five groups with 10 in each group. Group (1): control group. Group (2): rats injected with Doxorubicin (DOX) (3 mg/kg) and Cyclophosphamide (CYP) (50mg/kg) dissolved in sterile physiological saline injected I/P once a week for 5 weeks. Group (3): 10 rats undergo chemoablasion then injected with 5 IU of pregnant mare serum gonadotropin (PMSG) single s/c injection. Group (4): 10 rats undergo chemoablasion, then iPSc injected i/v single injection for 2 months. Group (5): 10 rats undergo chemoablasion then iPSc injected i/v combined with single s/c injection of 5 IU PMSG. Results revealed that rats injected with Dox and CYP showed asignificant decrease in RBCs, Hb and PCV, WBCs, lymphocytes, neutrophil, eosinophil, E2 level and Oct 4 while there was significant increase in MCV, MCH, monocytes and FSH level compared with control (-ve) group. While, rats treated with PMSG or IPSc or PMSG and IPSc revealed significant increase in RBCs count as well as MCHC, E2 level and Oct 4 with non significant changes in Hb and PCV, WBCs, lymphocytes, eosinophil with asignificant increase of neutrophil IPSc or PMSG and IPSc groups and asignificant decrease in monocytes compared with OA group and control (-ve) group.